Submitted: 12 Mar 2020
Revised: 06 Aug 2020
Accepted: 16 Aug 2020
First published online: 30 Aug 2020
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Int J Enteric Pathog. 2020;8(3):84-88.
doi: 10.34172/ijep.2020.19
  Abstract View: 134
  PDF Download: 166

Original Article

Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa

Amir Karimipour-Saryazdi 1 ORCiD, Fatemeh Ghaffarifar 1, Pooya Tavakoli 1, Yeganeh Karimipour-Saryazdi 2, Leila Zaki 1, Saeed Bahadory 1 * ORCiD

1 Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
*Corresponding Author: Saeed Bahadory, Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. E-mail: Email: Saeed.Bahadori@ modares.ac.ir

Abstract

Background: Acanthamoeba is a globally dispersed protozoan that can cause different clinical manifestations in infected individuals. Various drugs have been proposed against its drug-resistant forms.

Objective: The present study examined silver nanoparticles (NPs) with a good anti-parasitic background. More precisely, the study focused on evaluating the anti-parasitic effect of silver nano-scale particles on protozoan trophozoite and cysts by microscopic counting and flow cytometry after exposure to different concentrations.

Methods: To this end, MTT assay and IC50 were used to assess the macrophage toxicity and cysts/trophozoites, respectively.

Results: Based on the results, 100 ppm silver NPs had better anti-parasitic effects than 80 ppm concentration and even the standard treatment of Acanthamoeba on both trophozoite and cystic phases. Macrophages toxicity at 100 ppm concentration was similar to the control group.

Conclusion: In general, further studies should be conducted to confirm the present results given the significant effects of silver NPs against trophozoite and parasite cysts.



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