Submitted: 28 Jul 2019
Revised: 25 Aug 2020
Accepted: 27 Aug 2020
First published online: 30 Aug 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

Int J Enteric Pathog. 2020;8(3):76-79.
doi: 10.34172/ijep.2020.17
  Abstract View: 97
  PDF Download: 154

Original Article

Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran

Mahtab Hamidpour 1 ORCiD, Saman Mahdavi 2 * ORCiD

1 Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran
*Corresponding Author: Saman Mahdavi, Tel: +989144150454, Email: Email: s.mahdavi@iau-maragheh.ac.ir

Abstract

Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type.

Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran.

Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers.

Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively.

Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.


First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 97

Your browser does not support the canvas element.


PDF Downloaded: 154

Your browser does not support the canvas element.