Submitted: 02 Jul 2017
Revised: 16 Dec 2017
Accepted: 18 Dec 2017
First published online: 18 Dec 2017
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Int J Enteric Pathog. 2018;6(1):18-21.
doi: 10.15171/ijep.2018.05
  Abstract View: 1391
  PDF Download: 955

Original Article

Isolation of Yersinia enterocolitica Bacteriophage From Hospital Wastewater

Fatemeh Daneshgar 1, Mohammad Mehdi Soltan Dallal 2,3 * , Farzaneh Hosseini 4

1 Science and Research Branch, Islamic Azad University, Tehran Iran
2 Department of Medical Microbiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran
3 Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
4 Tehran North Branch, Islamic Azad University, Tehran Iran


Background: Yersiniosis is a common foodborne infection caused by Yersinia enterocolitica. This bacterium is frequently isolated from animals. Generally, bacteriophages (phages) are viruses that only infect bacteria, and are ubiquitous in the world including the intestinal tracts of animals and sewage. Today, due to the development of antibiotic resistance among bacteria, studies on phages have been considered as a controlling factor for bacterial infections.

Objective: The aim of this study was to identify and isolate Y. enterocolitica, the bacterium-specific phage, from raw sewage.

Materials and Methods: Every 10 minutes, 6 samples each containing 30 mL raw wastewater were collected from the sewage treatment center of Vali-asr hospital, Tehran-Iran. Bacteriophage was isolated from sewage samples using Double-Layer Agar method. In addition, the samples were purified and the volume required for the isolation of bacteriophage was determined. Then, we investigated the sensitivity of the isolated bacteriophage to the temperature.

Results: Lytic bacteriophages were isolated from the samples obtained from hospital sewage. This bacteriophage was largely active on Y. enterocolitica. It was active at 4, 22, 37, 40, 50, 60, and 70°C, however it became inactive at 80°C.

Conclusion: The results showed that Y. enterocolitica specific bacteriophage could be isolated from hospital sewage samples.

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