Submitted: 10 Mar 2017
Revised: 04 Jul 2017
Accepted: 18 Jul 2017
First published online: 24 Jul 2017
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Int J Enteric Pathog. 2017;5(3):70-75.
doi: 10.15171/ijep.2017.17
  Abstract View: 1848
  PDF Download: 1220
  Full Text View: 138

Original Article

The Inhibitory Effects of 2 Commercial Probiotic Strains on the Growth of Staphylococcus aureus and Gene Expression of Enterotoxin A

Mahnoosh Parsaeimehr 1 * , Maryam Azizkhani 2, Ashkan Jebelli Javan 1

1 Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
2 Food Hygiene Department, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
3Corresponding author: Mahnoosh Parsaeimehr ,Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran, TEL: +98-23- 33654215; FAX: +98-23- 33654214; Email: mparsaei@profs.semnan.ac.ir

Abstract

Background: Food-borne intoxications are current problems in human society and most of them are caused by the enterotoxins of Staphylococcus aureus. Staphylococcal enterotoxin A (SEA) is the most frequently responsible for staphylococcal food poisoning outbreaks. From a food safety and human health point of view, lactic acid bacteria (LAB) may provide a promising strategy to combat the pathogenic bacteria, particularly S. aureus.

Objective: The objective of this study was to evaluate the inhibitory activity of two commercial lactobacillus strains on growth and enterotoxin A production by S. aureus. Moreover, the inhibitory effect of these strains on gene expression of enterotoxin type A was assessed using real-time Polymerase chain reaction (PCR).

Materials and Methods: In this study the inhibitory effect of two commercial probiotic strains, Lactobacillus acidophilus (LA5) and Lactobacillus casei 01 on the growth and enterotoxin production of S. aureus was evaluated at 25 and 35°C. The gene expression of SEA of S. aureus was also evaluated by real time (RT) PCR technique.

Results: The lactobacillus strains decreased the bacterial count at both temperatures compared with the control group. This reduced effect was greater at 25°C (3 log/CFU) than 35°C (2 log/CFU). The production of SEA, SEC and SEE was inhibited by the lactobacillus strains. Furthermore, the gene expression of SEA was significantly suppressed in S. aureus co cultured with studied lactobacillus strains and the greatest down-regulation of sea (10.31 fold) was observed in co-incubation of S. aureus with LC01 at 25°C.

Conclusion: This research raises important implications for the potential use of LAB as a natural preservative in foodstuffs by correct microbial ecology of the environment and a new approach for biocontrol of S. aureus.

Copyright © 2017 The Author(s); Published by Alborz University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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