Submitted: 14 Oct 2015
Revised: 24 Nov 2015
Accepted: 01 Dec 2015
First published online: 07 Oct 2016
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Int J Enteric Pathog. 2016;4(2): e33806.
doi: 10.17795/ijep33806
  Abstract View: 1720
  PDF Download: 1067

Research Article

Plasmid Profile Analysis of Aminoglycoside-Resistant Escherichia coli Isolated From Urinary Tract Infections

Neda Soleimani 1 * , Safoura Derakhshan 2, Mojtaba Memariani 3

1 Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, IR Iran
2 Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
3 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
*Corresponding author: Neda Soleimani, Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, IR Iran. Tel: +98-2129905516, Email:


Background: Uropathogenic E. coli (UPEC) is the primary cause of human urinary tract infections (UTIs) worldwide. Moreover, there has been renewed and growing interest in using older antibiotics for treatment, such as aminoglycosides.

Objectives: The goal of this study was to determine the plasmid profile patterns of UPEC isolates harboring the aminoglycoside resistance gene aac(3)-IIa.

Patients and Methods: A total of 276 uropathogenic E. coli (UPEC) samples were isolated from UTI patients at the Tehran heart cente rin Tehran, Iran. Antimicrobial susceptibility testing against five aminoglycosides was performed by the disk diffusion method, and the aac(3)-IIa gene was detected via PCR. Moreover, plasmid profiling was carried out on those UPEC isolates harboring the aac(3)-IIa gene. Finally, the similarities among these isolates were determined on the basis of their plasmid profiles.

Results: The highest level of resistance was seen for tobramycin (24.6%), and the aac(3)-IIa gene was found in 51 isolates. Twentyseven different plasmid profiles were identified among the isolates harboring the aac(3)-IIa gene, with the 15 kb plasmid being the most common. Moreover, no significant correlation was found between the resistance patterns and the number of plasmids. The cluster analysis based on the plasmid profiles grouped the isolates into five different clusters, of which cluster one was the largest (containing 14 of 51 isolates).

Conclusions: Our data suggest the monitoring of aminoglycoside resistance, and its consideration in the empirical therapy of UPEC infections.

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