Submitted: 18 Sep 2019
Revised: 15 Nov 2019
Accepted: 15 Dec 2019
First published online: 28 Feb 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

Int J Enteric Pathog. 2020;8(1):19-24.
doi: 10.34172/ijep.2020.05
  Abstract View: 264
  PDF Download: 203

Original Article

Molecular Characterization of Methicillin-Resistant Enterotoxin-Producing Staphylococcus aureus Isolated From Samosa and Falafel in Iran

Saeed Khaledian 1 ORCiD, Mohammadreza Pajohi-Alamoti 1 * ORCiD, Pezhman Mahmoodi 2

1 Department of Food Hygiene and Quality Control, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
2 Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
*Corresponding Author: Mohammadreza Pajohi-alamoti, Department of Food Hygiene and Quality Control, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran Tel: +988134227350 , Fax: +988134227475 , Email:, pajohi@gmail.com Email: mr.pajohi@basu.ac.ir

Abstract

Objective: This study aimed to determine the contamination rate of Staphylococcus aureus in Samosa and falafel as most popular snacks, detect the classic enterotoxins, mecA, and tst genes and investigate antimicrobial resistance in the isolates.

Materials and Methods: The samples were examined using bacterial culture and the suspected isolates were characterized by biochemical tests. The identity of S. aureus isolates and the presence of enterotoxin-encoding genes were assessed using a multiplex polymerase chain reaction (PCR) assay and antibiotic resistance of the isolates was determined.

Results: The results revealed that 56 (46.67%) samples were contaminated with S. aureus, among which 45 isolates (80.35%) were characterized as enterotoxigenic S. aureus. The highest prevalence rate belonged to sea encoding gene as 20 isolates (35.71%) were positive for this gene followed by sed gene which was detected in 14 S. aureus isolates (25%). Most isolates (75%) were resistant to cefoxitin. Moreover, the results of PCR assays indicated that 10 (17.58%) and 7 (12.5%) isolates were positive for mecA and tst genes, respectively.

Conclusion: The results of the present study demonstrated that staphylococcal contamination of Samosa and falafel should be considered as a potential health risk for consumers.

First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 264

Your browser does not support the canvas element.


PDF Downloaded: 203

Your browser does not support the canvas element.