Submitted: 19 Oct 2019
Revised: 11 Oct 2019
Accepted: 04 Nov 2019
First published online: 21 Dec 2019
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Int J Enteric Pathog. 2019;7(4):121-125.
doi: 10.15171/ijep.2019.25
  Abstract View: 37
  PDF Download: 57

Research Article

Characteristics of Erythromycin Resistance in Methicillin-Resistant Staphylococcus aureus Isolated From Raw Milk

Fatemeh Mahdavi 1, Fatemeh Zaboli 2, Rahem Khoshbakht 3 * ORCiD

1 MSc Student of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are one of the most important multidrug resistant microorganisms that threaten human health.

Objective: The present study was conducted to evaluate genotypic and phenotypic characteristics of erythromycin resistance among MRSA isolates recovered from raw milk in Iran.

Materials and Methods: A total of 50 MRSA isolates were recovered from raw milk. Tests for erythromycin and clindamycin susceptibility and inducible clindamycin resistance were done. In addition, the presence of the methicillin resistance determinant (mecA), erythromycin resistance genes (ermA, ermB, ermC and msrA) and an important virulence gene (Panton– Valentine leukocidin) were investigated using polymerase chain reaction (PCR) method.

Results: Forty-eight percent (24/50) and 46% (23/50) of the isolates were resistant to erythromycin and clindamycin, respectively. Seven (14%) isolates showed inducible clindamycin resistance phenotype. The mecA gene was detected in 88% (44/50) of MRSA isolates. The incidence of the ermA, ermB, ermC and msrA genes was 14%, 64%, 12%, and 26%, respectively and the PVL gene was present in 18% (9/50) of MRSA isolates.

Conclusion: According to the results of the study, the incidence of erythromycin resistance genes and inducible clindamycin-resistant MRSA strains was high in raw milk samples in Iran.

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