Submitted: 05 Oct 2018
Revised: 26 Nov 2018
Accepted: 28 Nov 2018
First published online: 30 Nov 2018
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Int J Enteric Pathog. 2018;6(4):104-107.
doi: 10.15171/ijep.2018.26
  Abstract View: 204
  PDF Download: 161

Original Article

Investigating the Presence and Expression of stx1 Gene in Escherichia coli Isolated From Women With Urinary Tract Infection Using Real-Time PCR in Tabriz, Iran

Saman Mahdavi 1 * ORCiD, Elham Tanhaeivash 2, Alireza Isazadeh 3,4

1 Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran
2 Department of Genetic, Bonab Branch, Islamic Azad University, Bonab, Iran
3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
*Corresponding Author: Saman Mahdavi, Tel: +98-9144150454 Email: s.mahdavi@iau-maragheh.ac.ir

Abstract

Background: Urinary tract infection (UTI) is one of the most common diseases in all the age categories around the world.

Objective: The current study aimed to investigate the presence and expression of the stx1 gene in Escherichia coli isolated from women with UTI in Tabriz.

Materials and Methods: In this descriptive cross-sectional study, 25 E. coli isolates were assessed for the presence of the stx1 gene by polymerase chain reaction (PCR) method after DNA extraction. Then, they were evaluated in terms of stx1 gene expression by the real-time PCR.

Results: Six (24%) out of 25 tested E. coli isolates harbored stx1 gene. In addition, 2 samples out of these 6 isolates containing the stx1 gene showed more gene expression compared to the control samples.

Conclusion: Based on the prevalence of UTI with E. coli among the community and diffusion of its pathogenesis factors, fast and accurate identification of E. coli and its resistance factors is necessary for curring this infection.

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