Submitted: 04 Feb 2018
Revised: 24 Jan 2019
Accepted: 10 Feb 2019
First published online: 18 Feb 2019
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Int J Enteric Pathog. 2019;7(1):27-29.
doi: 10.15171/ijep.2019.07
  Abstract View: 151
  PDF Download: 146

Original Article

Genotype Characterization of Human Hydatid Cyst Isolates From Patients in Karaj, Iran, Using COX1 Gene Sequence

Maryam Salimi 1, Abolfazl Miahipour 1,2 * ORCiD, Mohammad Zibaei 1, Sasan Rezaie 3

1 Department of Medical Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
2 Dietary Supplements and Probiotics Research Center, Alborz University of Medical Sciences, Karaj, Iran
3 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: Cystic echinococcosis is a main zoonotic infection. It can cause serious clinical problems for human health around the world. Genotypic specification of Echinococcus granulosus in human is important due to control and prevention programs. Objective: In this investigation, genetic characteristics of human isolates of E. granulosus in Karaj, Iran, were studied. Materials and Methods: In this review, 3 isolates of surgically removed hydatid cysts were obtained from patients in Shahid Madani hospital, Karaj, Iran in 2014. DNA was extracted from the protoscolex of the cyst, and polymerase chain reaction (PCR) assay was done on the COX1 gene. Results: DNA fragments were sequenced and the results were aligned and analyzed. Among the isolates, 3 (100%) were E. granulosus (G1) strain. Conclusion: The G1 genotype was the most superior strain from human isolates of hydatid cyst in Karaj.
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