Mohammad Mehdi Soltan Dallal 1,2 
,
Freshteh Fani 3,
Zahra Rajabi 2,
Mohsen Karami-Talab 1,
Hedroosha Molla Agha mirzaei 2 *
1 Devision Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2 Food Microbiology Research Center,Tehran University of Medical Sciences, Tehran, Iran
3 Professor Alborzi Clinical Microbiology Research Center, Faculty of Medicine, Shiraz University of Medical Sciences, Fars, Iran
Abstract
Aim: In this study we focused on
evaluating the prevalence of ESBL enzymes genes
that are including CTX-M, SHV, TEM β-lactamase among E.
coli isolates in
foodborne outbreak.
Background: Extended
Spectrum Beta-Lactamase (ESBL) has known as
enzymes that are responsible for creating of high level of antimicrobial
resistance in gram negative microorganism especially in E.coli. -Therefore, survey and identify of generating genes of
these enzymes can be effective in
controlling of resistance and helpful in
using of appropriate βlactam drug against them.
Material and Methods: Five hundredstool samples are including diarrhea with nausea, vomiting, abdominal cramps, fever and headache
were selected by IMVIC biochemical test for E.coli
. By making antimicrobial susceptibility test
base on CLSI and using disk diffusion
method and confirmatory test (combined disk) ,ESBLs samples were determined. Subsequently polymerase chain reaction (PCR) were
performed for the following relative genes.
Result: The highest - resistance in E. coli isolates was related to ampicillin (56%) -ESBLs positive were showed in (36%) samples , after PCR prosseccing the prevalence of SHV, TEM and CTX-M1 genes was (0%) , (18%) and (38%) respectively.
Conclusions: Due to the prevalence of β-lactamase genes , accurate and rapid identification methods as PCR assay is essential. So, this can be considered as routine methods -