Submitted: 20 May 2017
Revised: 16 Jul 2017
Accepted: 11 Aug 2017
First published online: 21 Aug 2017
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Int J Enteric Pathog. 2017;5(3):80-84.
doi: 10.15171/ijep.2017.19
  Abstract View: 601
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Original Article

Effect of Essential Unsaturated Fatty Acids on Structure and Function of Catalase at High Glucose Concentration

Hossain Mirmiranpoor 1,2 * , Payam Hashemi 2, Fatemeh Dehghani Firouzabadi 2, Niloofar Alishiri 3, Mitra rahimzadeh 4

1 Dietary Supplements and Probiotics Research Center, Alborz University of Medical Sciences, Karaj, Iran
2 Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Deputy of Research Affairs, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
4 Research Center for Social Determination of Health, Alborz University of Medical Sciences, Karaj, Iran

Abstract

Background: Assessment of changes in structure and activity of catalase, as an antioxidant enzymatic protein, in combination with glucose and essential unsaturated fatty acids is the aim of present study.

Materials and Methods: In order to investigate the activity and structure of catalase, a solution of this enzyme with 10 mg/mL concentration in phosphate buffer (0.1 M) and pH = 7.4, in the presence and absence of 50 mmol glucose was prepared, filtered, and then incubated for 4 months at 37°C with and without fatty acids including linolenic, linoleic, and arachidonic acids, separately. Samples were taken from each tube of solution, every 14 days for 4 months, to assess the fluorescence emission, circular dichroism (CD) and activity.

Results: According to the results, catalase showed increase in fluorescence emission and decrease in activity after incubation with glucose in comparison with pure protein. Moreover, catalase showed alteration in CD after incubation with glucose during 4 months. After incubation with glucose and each of the mentioned unsaturated fatty acids, alterations of catalase were neared toward normal level in fluorescence emission, CD, and activity in comparison with pure protein.

Conclusion: Functional and structural protection of catalase against damages from hyperglycemic environment with addition of essential unsaturated fatty acids was proved in our investigation.

Copyright © 2017 The Author(s); Published by Alborz University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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