Submitted: 01 Oct 2016
Accepted: 01 Oct 2016
First published online: 15 Nov 2016
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Int J Enteric Pathog. 2016;4(4): e37105.
doi: 10.15171/ijep.2016.15
  Abstract View: 565
  PDF Download: 494
  Full Text View: 588

Original Article

Antimicrobial Resistance Characteristics and Phylogenetic Groups of Escherichia coli Isolated From Diarrheic Calves in Southeast of Iran

Zahedeh Naderi 1 * , Reza Ghanbarpour 1, Masoud Sami 2,3

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IR Iran
2 Department of Food Sciences and Technology, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, IR Iran
3 Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran
*Corresponding author: Zahedeh Naderi 22 Bahman Blvd, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Tel: +98-9133455603 Fax: +98-3433257447 E-mail: Email: vetmicgroup@gmail.com

Abstract

Background: Antimicrobial resistance is one of the main challenges in diarrheal diseases in
human and animals. Regardless to the main reason of the disease, approximately all
antimicrobial actions including treatment, control and prevention are mostly centralized against
Escherichia coli (E. coli) strains.

Objectives: This work purposed to antimicrobial resistance (AR) and determinate virulence
genes and phylogenetic groups in E. coli isolates (n=170) obtained from calves with diarrhea.

Materials and methods: Isolates were molecular characterized for 17 AR genes and 3
phylogenetic sequences. AR phenotyping were performed on all strains for 12 antimicrobial
agents by using disc diffusion method.

Results: All AR genes but qnrS were identified with different prevalence in E. coli isolates that
the most common genes were aadA (20%), blaTEM (11.7%) and sulII (11.2 %) belonging to
aminoglycoside, β-lactamase and sulphonamide families, respectively. Resistance to the
penicillin and sulphamethoxazole drugs was found in 100% of isolates and followed by
tetracycline (73.5%), streptomycin (60%), trimethoprim sulphamethoxazole (56.5%) and
kanamycin (53.5%). The phylogenetic groups A and B1 considerably surrounded the majority of
isolates with the frequency of 65.8% and 30.6%, respectively.

Conclusions: In Iran, diarrheic calves have an important role as reservoir of resistant E. coli
strains against the some drugs which are registered for treatment of calf diarrhea.

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