Submitted: 01 Oct 2016
Accepted: 01 Oct 2016
First published online: 15 Nov 2016
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Int J Enteric Pathog. 2016;4(4): e37106.
doi: 10.15171/ijep.2016.16
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Original Article

Evaluation of Colicin Effect on the Induction of Treated Mice in Prevention of Infection Caused by Escherichia coli K99

Yahya Tahamtan 1 * , Fatemeh Golestan 2, Elham Moazamian 3

1 Bacteriology Department, Razi Vaccine and Serum Research Institute, Shiraz Branch, Iran
2 Microbiology Department, Azad University of Jahrom, Jahrom, Iran
3 Microbiology Department, Azad University of Shiraz, Shiraz, Iran
Corresponding author: YahyaTahamtan, Razi Vaccine and Serum Research Institute, Shiraz, Iran. Phone: +987136240331, Fax: +987136240201, Email:, Email:


Background. Colicin produce by colicinogenic E. coli (CEC) arenarrow limited
spectrum antimicrobial agents that are able to kill or prevent close related strains.
Objective. The objective of this study was to evaluation effect of Colicin to induce
immunized mice to prevent infection caused by E. coliK99.

Patient and Methods. The experiment was conducted into two mice groups (30 in
each group) with two weeks old. All mice were administered by streptomycin sulfate
prior to treatment to eliminate resident E. coli. Group one was orally inoculated with
PBS as control and the second was immunized by Colicin solution as immunize
group. Both control and immunized group were challenged by 3 LD 50 of E. coli K99
and follow a week.

Results. Immunized mice group were not showed severe clinical signs. While
diarrhea with different sings of colibaccillosis was established in control group and
infected mice was died.

Conclusion. Overuse antibiotics developed serious new types of multi drug resistance
in human medicine and therefore has limited their use in farm animals. The study
indicates the use of Colicin and biotherapy instead of antibiotic is more safe and
efficient for control of E. coliK99 infection. Immunized mice by Colicin solution
protected E. coli K99 colonization and reduce fecal shedding. Investigation in
livestock for applying Colicin in farm animal is recommended.

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