Submitted: 15 Nov 2014
Revised: 30 Nov 2014
Accepted: 21 Jan 2015
First published online: 06 Oct 2016
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Int J Enteric Pathog. 2015;3(2): e25366.
doi: 10.17795/ijep25366
  Abstract View: 658
  PDF Download: 389

Research Article

Antibiotic Resistance Profile in Relation to Phylogenetic Background in Escherichia coli Isolated From Fecal Samples of Healthy Ostrich

Elham Mohamadi 1, Hesam Alizade 2 * , Nasrin Askari 1, Mahmood SalehI 1, Mitra Porjafarian 1, Reza Ghanbarpour 1,3

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, IR Iran
2 Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, IR Iran
3 Zoonosis Research Committee, Kerman University of Medical Sciences, Kerman, IR Iran
*Corresponding author: Hesam Alizade, Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, IR Iran. Tel: +98-9132456562, Fax: +98-3412112794, Email: alizade.h2000@yahoo.com

Abstract

Background: E. coli is regarded as a reservoir for antibiotic resistance in foods of animal origin. E. coli can be categories into four mainphylogenetic groups (A, B1, B2 and D). The commensal E. coli strains mostly are assigned to the phylo-groups A and B1.

Objectives: The purposes of this study were to determine the phylogenetic group/subgroups and antibiotic resistance patterns of ostrich E. coli isolates in Iran.

Materials and Methods: A total of 126 E. coli isolates were obtained from cloacae swabs of the healthy ostrich in Kerman, Iran. The E. coliisolates were confirmed using biochemical API 20E identification system. The confirmed isolates were studied to determine phylogeneticbackground by PCR. The isolates were tested for antibiotic resistance against 12 different antibiotic disk by disk diffusion method.

Results: Phylotyping of E. coli isolates indicated that 74 isolates belonged to A, 27 isolates to B1, 7 isolates to B2, and 18 isolates to D groups.Also the isolates fell into six phylogenetic subgroups, including 34 isolates in A0, 40 isolates in A1, one isolate in B22, 6 isolates in B23, 11isolates in D1 and 7 isolates in subgroup D2. In the examined E. coli isolates, the maximum rate of resistance was against tetracycline, andthe minimum rate of resistance was against amoxicillin. Twenty three antibiotic resistance patterns were detected among the isolates. Thecefoxitin and tetracycline resistance pattern was the most prevalent in the isolates that belonged to phylo-group A.

Conclusions: In conclusion, the result of the present study revealed a low frequency of antibiotic resistance in ostrich E. coli isolates.The antibiotic resistance patterns were in relation to A and D phylogenetic groups. Further studies are needed to better understand thedistribution of phylogenetic groups in poultry isolates.

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