Submitted: 23 Jun 2014
Revised: 10 Aug 2014
Accepted: 17 Aug 2014
First published online: 06 Oct 2016
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Int J Enteric Pathog. 2014;2(4): e21485.
doi: 10.17795/ijep21485
  Abstract View: 1122
  PDF Download: 698

Research Article

Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Narges Golab 1, Pejvak Khaki 2 * , Fatemeh Noorbakhsh 1

1 Department of Microbiology, Biology Faculty, Varamin Branch, Islamic Azad University, Varamin, IR Iran
2 Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, IR Iran
Corresponding author: Pejvak Khaki, Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, IR Iran. Tel: +98-9123894986, Fax: +98-2634552194, Email: 2p.khaki@rvsri.ac.ir

Abstract

Background: Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Poultryand Poultry products have been identified as one of the important foodborne sources of Salmonella. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations.

Objectives: The aim of this study was to carry out molecular typing of Salmonella enterica spp. by PFGE technique.

Materials and Methods: All 47 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE.

Results: In the current work, PFGE and serotyping were used to subtype 47 Salmonella isolates belonging to 22 different serotypes and derived from poultry. Thirty-nine PFGE patterns out of 47 isolates were obtained. The Discrimination Index (DI) by serotyping (0.93) was lower than PFGE (DI = 0.99).

Conclusions: In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes.

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