Submitted: 05 May 2014
Revised: 25 May 2014
Accepted: 02 Jun 2014
First published online: 06 Oct 2016
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Int J Enteric Pathog. 2014;2(4): e20012.
doi: 10.17795/ijep20012
  Abstract View: 783
  PDF Download: 522

Research Article

Mutagenicity Survey and Antibacterial Activity of  Various Generations of Poly (Amid Amine) (PAMAM) Dendrimers and Algonac Acid Poly (Amid Amine) (PAMAM) Dendrimer Nano Composite G2 on Some Enteric Pathogenic Bacteria

Babak Shahbazi 1, Mazaher Khodabandelo 2, Mohammad Jafar Rezaei 3, Samaneh Rouhi 1, Pegah Shakib 1, Rashid Ramazanzadeh 2 *

1 Department of Microbiology, Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
2 Cellular and Molecular Research Center, Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
3 Cellular and Molecular Research Center, Department of Anatomy, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
*Corresponding author: Rashid Ramazanzadeh, Cellular and Molecular Research Center, Department of Anatomy, Student Research Committee, Kurdistan university of Medical Sciences, Sanandaj, IR Iran. Tel: +98-8716131411, Fax: +98-8716664674, Email: atrop_t51@yahoo.com

Abstract

Background: Nano composites are widely used in medical sciences recently. Identification of chemical mutagens is an important issue in drug safety.

Objectives: The aim of this study was to investigate mutagenicity and antibacterial activity of various generations of poly (amid amine) (PAMAM) dendrimers and agonic acid poly (amid amine) (PAMAM) Nano composite G2 on enteric pathogenic bacteria.

Materials and Methods: Disc diffusion method, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) determination were performed for antibacterial activity survey. Mutagenic properties were determined by Ames assay.

Results: Various dilutions of PAMAM generations (G2, G3, G4 and G5) and agonic acid PAMAM Nano composite G2 had antibacterial effect on Escherichia coli, Salmonella enterica, Salmonella typhimurium, Enterococcus faecalis and Staphylococcus aureus. In Ames assay, reverted colonies were increased by PAMAM generations increasing. Moreover, reverted colonies Ames assay were lower when Nano composite G2 was applied.

Conclusions: PAMAM generations and agonic acid PAMAM Nano composite G2 contain compounds with therapeutic potential and antibacterial characteristics, which can be used in medicine. Because some chemicals have mutagenic and carcinogenic properties, identification of these chemical is of great importance.

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