Submitted: 28 Sep 2013
Revised: 05 Apr 2014
Accepted: 01 May 2014
First published online: 05 Oct 2016
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Int J Enteric Pathog. 2014;2(3): e18632.
doi: 10.17795/ijep18632
  Abstract View: 958
  PDF Download: 1040

Research Article

Clostridium perfringens Type D Epsilon Prototoxin and Toxin Effects on the Mouse Body Weight

Reza Pilehchian Langroudi 1 *

1 Department of Animal Anaerobic Bacteriology and Vaccine Production, Razi Vaccine and Serum Research Institute, Karaj, IR Iran
*Corresponding author: Reza Pilehchian Langroudi, Department of Animal Anaerobic Bacteriology and Vaccine Production, Razi Vaccine and Serum Research Institute, Karaj, IR Iran. Tel: +98-2634502720, Fax: +98-2634552194, Email: r.pilehchian@rvsri.ac.ir

Abstract

Background: Clostridium perfringensis one of the most important pathogens in human and animals and protection against this bacteria in domestic animals will be only achieved by vaccination, otherwise, high mortality rate and great financial burden will be issued.

Objectives: We aimed to evaluate the effects of C. perfringens Type D prototoxin and toxin on the mouse body weight.

Materials and Methods: After preparation of filtrate and freeze dried crude prototoxin, three series of experiments were set up. At the first step, minimum lethal dose per milliliter (MLD/mL) was determined and according to MLD, 50% endpoint (LD50) was determined. Finally, mice with 18 to 20 g body weight were injected with the different concentrations of activated freeze dried prototoxin.

Results: Two days after injection, a decrease in body weight was observed in experimental groups, while no decrease in body weight was observed in the control group. The results indicated that the activated C. perfringens culture filtrate temporarily inhibits mouse general metabolism.

Conclusions: As secreted prototoxin is activated in the small intestine of infected animals, vaccination of the domestic animals in the proper time by the appropriate vaccine could prevent these effects.

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