Submitted: 11 Dec 2013
Revised: 10 Feb 2014
Accepted: 16 Feb 2014
First published online: 05 Oct 2016
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Int J Enteric Pathog. 2014;2(2): e16749.
doi: 10.17795/ijep16749
  Abstract View: 994
  PDF Download: 690

Research Article

Antibacterial Activity of Garlic (Allium sativum L.) on Multi-Drug Resistant Helicobacter pylori Isolated From Gastric Biopsies

Fozieh Jahani Moghadam 1, Tahereh Navidifar 2, Mansour Amin 1 *

1 Department of Microbiology, School of Medicine, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, IR Iran
2 Department of Microbiology, Shahid Sadoghi University of Medical Sciences, Yazd, IR Iran
*Corresponding author: Mansour Amin, Department of Microbiology, School of Medicine, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, IR Iran. Tel: +98-9163051096, Email: mnsamin@yahoo.com

Abstract

Background: Garlic (Allium sativum L.) exhibit a broad-spectrum of antimicrobial activity against both gram negative and gram-positive bacteria. Helicobacter pylori (H. pylori) is the main factor in peptic and duodenal ulcer diseases. Some strains of H. pylori have becom eresistant to the current antibiotics.

Objectives: The purpose of this study was to determine the antibacterial effects of garlic on H. pylori.

Materials and Methods: The gastric biopsies were inoculated on Brucella agar base (Conda Pronadisa, Spain) supplemented with 5% sterile sheep blood, 7 % fetal calf serum, vancomycin (5 mg/L), trimethoprim (5 mg/L), and polymyxin B (2500 U/L). Antibiotic susceptibility to garlic and commercial antibiotic disc was determined by agar disc diffusion method.

Results: In this study, ten strains of H. pylori out of 120 samples were isolated. The minimum inhibitory concentration of garlic extract for all isolates ranged between 25- 400 mg/mL. Resistance rates of 10 strains of H. pylori to amoxicillin, ciprofloxacin, clarithromycin, metronidazole, and tetracycline were 20%, 20%, 30%, 60%, and 30%, respectively.

Conclusions: In this study, the antibacterial effect of aqueous extract of garlic on all H. pylori clinical isolates was confirmed. These findings might help us to use a new strategy for treatment of peptic ulcer.

Implication for health policy/practice/research/medical education:

In this study antimicrobial effect of the garlic extract on multi-drug resistant Helicobacter pylori isolated from gastric biopsy of patients referred to Imam Khomeini hospital in Ahvaz city- Iran was evaluated.

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