Submitted: 17 Oct 2013
Revised: 27 Oct 2013
Accepted: 10 Nov 2013
First published online: 05 Oct 2016
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Int J Enteric Pathog. 2013;1(2): e15466.
doi: 10.17795/ijep15466
  Abstract View: 664
  PDF Download: 359

Research Article

Risk of cagA DNA in H. Pylori Patients

Davoud Esmaeili 1 * , Saeideh Hatami 2, Abbas Bahador 3

1 Applied Microbiology Research Center and Department of Microbiology, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
2 Department of Biology Tehran, Science and Research Branch, Islamic Azad University, IR Iran
3 Department of Medical Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
*Corresponding author: Davoud Esmaeili, Baqiyatallah University Medical of Sciences, Tehran, IR Iran. Tel: +98-2188067969, Fax: +98-2188039883, Email: esm114@gmail.com

Abstract

Background: Infection of Helicobacter pylori exists all around the world. This bacterium has an IV type secretion system. The main objective of this study was to investigate the existence and abundance of cagA gene in biopsy and serum samples by applying the PCR technique and assay of Triglyceride and cholesterol level in sera.

Objectives: The aim of this study was to determine the correlation between the presence of cagA genome and cardiac risk markers in infected patients with H. pylori.

Patients and Methods: 100 serum samples of patients with above IgG titer against Helicobacter pylori were examined with PCR to investigate the existence of cagA gene. Moreover triglyceride and cholesterol titers and blood pressure were measured.

Results: Eighteen samples out of 100 positive serumic samples from patients with helicobacter pylori had a positive result for the existence of cagA gene. Twelve samples (66٪) out of eighteen serumic samples had triglyceride and cholesterol titer greater than the normal levels. From 18 specimens detected in sera, about 12 people had cardiac disorders and 10 patients had high blood pressure.

Conclusions: Since secretion system of type IV is capable of secreting both genome and the protein of this bacterium into the cell, we decided to investigate the existence of cagA gene in sera. This bacterium was unable to induce septicemia and bacteriemia; the possibility is that the gene integrated with protein cagA to blood, was protected from degradation which increases the risk of antibody production against these factors and elevates the risk of heart disease. For this reason, for the first time in the world, we studied the presence of cagA genome in serum samples.

Implication for health policy/practice/research/medical education:

The aim of this study was to determine the correlation between the presence of cagA genome and cardiac risk markers in infected patients with H. pylori.

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