Farzaneh Firoozeh 1,
Fereshteh Shahcheraghi 2 * ,
Taghi Zahraei-Salehi 3,
Mohammad Mehdi Aslani 2,
Reihaneh Banisaeed 31 Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran
2 Department of Microbiology and Microbiology Research Center, Pasteur Institute, Tehran, IR Iran
3 Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IR Iran
Corresponding author: Fereshteh Shahcheraghi, Department of Microbiology and Microbiology Research Center, Pasteur Institute, Tehran, IR Iran. Tel: +98-2166405535, Fax: +98- 2166405535,
Email: shahcheraghifereshteh@yahoo.com
Abstract
The frequency of multidrug-resistance (MDR) among Salmonella isolates has increased dramatically. Here we report the first case of CTX-M type ß–lactamase producing ciprofloxacin-resistant Salmonella infection as a child in Iran. Antimicrobial resistance profile was determine daccording to the Clinical and Laboratory Standards Institute. A double disk synergy test was used in the production of extended-spectrum β-lactamases (ESBLs). PCR assays were carried out to detect blaTEM, blaSHV and blaCTX-M beta lactamase genes. The presence of class 1 integrons was investigated by PCR assays. The Salmonella Enteritidis isolate from the child was ciprofloxacin and extended-spectrum cephalosporins resistant and found to carry the blaCTX-M beta-lactamase gene and class 1 integrons, suggests that antimicrobial resistance determinants may be located on the same genetic unit of the class 1 integrons identified here. Resistance to ciprofloxacin and extended spectrumcephalosporins, the drugs of choice for treating invasive salmonellosis, especially in children is a public health problem.