Submitted: 28 Sep 2017
Revised: 13 Dec 2017
Accepted: 19 Dec 2017
First published online: 23 Dec 2017
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Int J Enteric Pathog. 2018;6(1):27-30.
doi: 10.15171/ijep.2018.07
  Abstract View: 135
  PDF Download: 119

Original Article

Molecular Typing of Klebsiella pneumoniae isolates using Repetitive Extragenic Palindromic Sequence-Based PCR in a Hospital in Tehran, Iran

Abdolmajid Ghasemian, Morvarid Shafiei 3, Majid Eslami 4, Mahtab Vafaei 1, Farshad Nojoom 1, Hassan Rajabi-Vardanjani 5 *

1 Microbiology Department, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
2 Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran
3 Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
4 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5 Researcher of Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Background: The presence of extended-spectrum β-lactamases (ESBLs) is increasing worldwide and blaCTX-M1 is the predominant β-lactamase.

Objective: This study was conducted to determine the ESBL production and prevalence of blaCTX-M1, blaSHV and blaTEM and AmpC genes and repetitive extragenic palindromic polymerase chain reaction (rep-PCR) pattern among Klebsiella pneumoniae isolates in Tehran from 2014 to 2016.

Materials and Methods: One hundred eleven isolates were collected during the study period. The PCR was employed to detect the blaCTX-M1, blaSHV, blaTEM and AmpC genes. The genetic relation of isolates was performed using rep-PCR typing method.

Results: Eighty-three and 86 isolates showed Minimum inhibitory concentration (MIC) ≥2 against ceftazidime and cefotaxime, respectively and 80 (72%) isolates exhibited ESBL production. The prevalence of blaCTX-M1, blaSHV, blaTEM and AmpC genes among ESBL producers was 92.5% (n = 74), 66.2% (n = 53), 56.2% (n = 45) and 2.5% (n = 2), respectively. The rep-PCR typing pattern of isolates showed a wide diversity, indicating the polyclonal spread of CTX-M type producing isolates.

Conclusion: The findings of this study highlighted the emergence and spread of K. pneumoniae isolates producing CTX-M and other ESBL enzymes with diverse genetic backgrounds in a hospital in Tehran.

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