Submitted: 14 Aug 2017
Revised: 10 Jan 2018
Accepted: 15 Jan 2018
First published online: 27 Feb 2018
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Int J Enteric Pathog. 2018;6(2):48-52.
doi: 10.15171/ijep.2018.13
  Abstract View: 248
  PDF Download: 187

Original Article

Prevalence of blaCTX-M, blaSHV and blaTEM β-Lactamase Genes Among Escherichia coli Isolates in Foodborne Outbreak in Iran

Mohammad Mehdi Soltan Dallal 1,2 ORCiD, Freshteh Fani 3, Zahra Rajabi 2, Mohsen Karami-Talab 1, Hedroosha Molla Agha mirzaei 2 *

1 Devision Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2 Food Microbiology Research Center,Tehran University of Medical Sciences, Tehran, Iran
3 Professor Alborzi Clinical Microbiology Research Center, Faculty of Medicine, Shiraz University of Medical Sciences, Fars, Iran

Abstract

Aim: In this study we focused on evaluating the prevalence of ESBL enzymes genes that are including CTX-M, SHV, TEM β-lactamase among E. coli isolates in foodborne outbreak. Background: Extended Spectrum Beta-Lactamase (ESBL) has known as enzymes that are responsible for creating of high level of antimicrobial resistance in gram negative microorganism especially in E.coli. -Therefore, survey and identify of generating genes of these enzymes can be effective in controlling of resistance and helpful in using of appropriate βlactam drug against them. Material and Methods: Five hundredstool samples are including diarrhea with nausea, vomiting, abdominal cramps, fever and headache were selected by IMVIC biochemical test for E.coli . By making antimicrobial susceptibility test base on CLSI and using disk diffusion method and confirmatory test (combined disk) ,ESBLs samples were determined. Subsequently polymerase chain reaction (PCR) were performed for the following relative genes. Result: The highest - resistance in E. coli isolates was related to ampicillin (56%) -ESBLs positive were showed in (36%) samples , after PCR prosseccing the prevalence of SHV, TEM and CTX-M1 genes was (0%) , (18%) and (38%) respectively. Conclusions: Due to the prevalence of β-lactamase genes , accurate and rapid identification methods as PCR assay is essential. So, this can be considered as routine methods -
Keywords: Escherichia coli, TEM, CTX-M, SHV, PCR
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