Submitted: 05 Dec 2015
Revised: 18 Feb 2016
Accepted: 01 Mar 2016
First published online: 23 Apr 2016
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Int J Enteric Pathog. 2016;4(3): e35249.
doi: 10.15171/ijep.2016.02
  Abstract View: 442
  PDF Download: 368
  Full Text View: 242

Original Article

Typing Toxigenic Clostridium perfringens Strains from the Ruminants of Yazd Province by Multiplex Polymerase Chain Reaction

Majid Ezatkhah 1, Mojtaba Alimolaei 1, Maryam Amini 1, Mehrdad Shamsaddini Bafti 1 *

1 Molecular Microbiology Department, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, IR Iran
*Corresponding Author: Mehrdad Shamsaddini Bafti, Tel: +98-9133980638; Fax: +98-34 32233051; Email: m.shamsaddini@rvsri.ac.ir

Abstract

Background: Anaerobic bacterial infections, especially enterotoxemia, are common ruminant disorders.

Objectives: The purpose of this study was to identify the toxigenic isolates of Clostridium perfringens in the ruminants of Yazd province.

Materials and Methods: In total, 485 fecal and intestinal samples were obtained and analyzed for typing C. perfringens toxovars by multiplex polymerase chain reaction (PCR). Only 179 bacterial strains passed the biochemical tests and 87 C. perfringens strains were confirmed by multiplex PCR.

Results: Interestingly, the predominant C. perfringens toxovar was type A (89.7%), while type D (9.2%) was also identified as a pathogen in the ruminants of Yazd province.

Conclusions: Detection of toxigenic C. perfringens isolates with multiplex PCR was performed for the first time in this field. The multiplex PCR used in this study provides a useful and reliable tool for C. perfringens genotyping in routine veterinary diagnostics,a nd epidemiological studies of the prevalent types of C. perfringens in Iran are possible by this technique. Genotyping of C. perfringens is recommended before starting vaccination programs.

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