Submitted: 24 Jan 2015
Revised: 14 Feb 2015
Accepted: 23 Feb 2015
First published online: 06 Oct 2016
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Int J Enteric Pathog. 2015;3(2): e27243.
doi: 10.17795/ijep27243
  Abstract View: 287
  PDF Download: 198

Research Article

Detection of Egg Yolk Immunoglobulin Y; a Potential Source of Anti- Escherichia coli

Enayatollah Kalantar 1,2, Mohammad Mehdi Soltan Dallal 2, Laya Kafami Khorasani 3, Koursh Kabir 4, Monireh Zenolabedini Zamani 2 *

1 Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, IR Iran
2 Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, IR Iran
4 Department of Social Medicine, School of Medicine, Alborz University of Medical Sciences, Karaj, IR Iran
*Corresponding author: Monireh Zenolabedini Zamani, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. Tel: +982188992971, Fax: +982188992971, Email: manasept63@gmail.com

Abstract

Background: E. coli strains can cause a wide variety of intestinal and extra-intestinal diseases. Increasing occurrence of antibioticresistance would complicate the treatment of such diseases.

Objectives: The aim of this study was to detect the egg yolk immunoglobulin Y as a potential source of anti-Escherichia coli.

Materials and Methods: This study was conducted by using killed E.coli with formaldehyde as antigen. Chickens were immunized with500 μL of antigen diluted in equal volume of Freund’s complete adjuvant (FCA). Booster injections of increasing concentrations of antigenwere given to raise the antibody level in serum. The specificity of antibody was confirmed by ELISA method.

Results: Chicken egg yolk antibodies (IgY) were raised against E. coli in the serum after injections. Up to 1.10000 dilution antibodies weredetermined in serum, which shows high amount of antibodies are produced in immunized chickens.

Conclusions: Further studies have to be conducted to evaluate the potency of these antibodies in treatment of variety of diseases. Theoutcome of this research work would be an alternative to the current antibiotic treatments.

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