Submitted: 13 Jul 2014
Revised: 21 Sep 2014
Accepted: 22 Sep 2014
First published online: 06 Oct 2016
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Int J Enteric Pathog. 2015;3(1): e22020.
doi: 10.17795/ijep22020
  Abstract View: 294
  PDF Download: 232

Research Article

Evaluation of Cholera Toxin Expression in Acidic, Alkaline and Neutral Conditions

Narges Rahimi 1, Mahnaz Nourani 2, Yousef Yahyapour 3, Enayatollah Kalantar 4, Sommayeh Yasliyanifard 4, Seyed Mahmoud Amin Marashi 5,6 *

1 Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, IR Iran
2 Infection Diseases Research Center, Babol University of Medical Sciences, Babol, IR Iran
3 Infectious Disease and Tropical Center, Babol University of Medical Sciences, Babol, IR Iran
4 Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, IR Iran
5 Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, IR Iran
6 Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, IR Iran
*Corresponding author: Seyed Mahmoud Amin Marashi, Department of Microbiology, Babol University of Medical Sciences, Babol, IR Iran. Tel: +98-1132910322, Email: parsmicrob@gmail.com

Abstract

Background: Cholera is a severe disease which is caused by Vibrio cholerae and it is typically transmitted by either contaminated food or water particularly in developing countries. The most important virulence factor of this bacterium is an enterotoxin called cholera toxin which is a protein complex secreted by the Vibrio cholerae.

Objectives: In this project, we determined the production of cholera toxin at different pH values.

Materials and Methods: Two standard strain of Vibrio cholerae O1 biovar EL Tor N16961 and Vibrio cholerae O1 biovar Classic ATCC 14035 were used. After overnight cultivation of both the strains the total mRNA extracted and converted to total cDNA.

Results: By Relative Real-Time PCR analysis the most cholera toxin production in classical and El Tor strains was at pH 8.5 and 8, respectively.

Conclusions: Therefore, We may conclude that use of acidic diet will help in reduction of cholera toxin production.

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