Submitted: 08 Jul 2014
Revised: 04 Sep 2014
Accepted: 20 Sep 2014
First published online: 06 Oct 2016
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Int J Enteric Pathog. 2015;3(1): e21840.
doi: 10.17795/ijep21840
  Abstract View: 255
  PDF Download: 179

Research Article

Evaluation of Cholera Toxin Expression in Different Populations of Vibrio cholera

Sedigheh Ebrahimi Kasgari 1, Mahnaz Nourani 2, Yousef Yahyapour 3, Seyed Ehsanollah Mousavi 4, Enayatollah Kalantar 5, Hami Kaboosi 1, Seyed Mahmoud Amin Marashi 5,6 *

1 Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, IR Iran
2 Infection Diseases Research Center, Babol University of Medical Sciences, Babol, IR Iran
3 Infectious Disease and Tropical Center, Babol University of Medical Sciences, Babol, IR Iran
4 Department of Microbiology, Babol University of Medical Sciences, Babol, IR Iran
5 Dietary and Probiotic Reserach Center, Alborz University of Medical Sciences, Karaj, IR Iran
6 Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, IR Iran
*Corresponding author: Seyed Mahmoud Amin Marashi, Department of Microbiology, Babol University of Medical Sciences, Babol, IR Iran. Tel: +98-1132910322, Email: parsmicrob@gmail.com

Abstract

Background: Cholera is one of the most diseases of human. Cholera toxin is the most important pathogenic factor in humans that causes diarrhea. The cholera toxin is produced by V. cholerae and CTXфPhage.

Objectives: In this study, we have investigated the production cholera toxin with different density of Vibrio cholerae.

Materials and Methods: With this propose we inoculated classical strain O1 of Vibrio cholerae ATCC 14035 and Vibrio cholerae O1biovar El Tor N16961 into the AKI medium. Then, the total mRNA was determined by standard procedure which was converted into total cDNA.

Results: Cholra toxin production was determined by qPCR and maximum production of cholera toxin was at 1010 cfu/mL.

Conclusions: In conclusion, production of cholera toxin was minimized almost up to zero at 1010.5 cfu/mL; which could be due to presence of high level concentration of autoinducer.

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