Submitted: 30 Jan 2014
Revised: 20 Feb 2014
Accepted: 26 Feb 2014
First published online: 05 Oct 2016
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Int J Enteric Pathog. 2014;2(2): e17928.
doi: 10.17795/ijep17928
  Abstract View: 456
  PDF Download: 307

Research Article

The Antibacterial Activity of Silver Nanoparticles Produced in the Plant Sesamum indicum Seed Extract: A Green Method Against Multi-Drug Resistant Escherichia coli

Mohammad Bokaeian 1, Mousa Sheikh 2, Mehdi Hassanshahian 3 * , Saeide Saeidi 4, Shahla Sahraei 5

1 Infectious Disease and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
2 Department of Microbiology, Medical Faculty, Kerman University of Medical Science, Kerman, IR Iran
3 Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, IR Iran
4 Department of Microbiology, College of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, IR Iran
5 Zabol University of Medical Sciences, Zabol, IR Iran
*Corresponding author: Mehdi Hassanshahian, Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran. Tel: +98-9132906971, Fax: +98- 3413222032, Email: mshahi@uk.ac.ir

Abstract

Background: The nanoparticles synthesis through biological processes is evolving a new era of research interests in nanotechnology. In usual methods silver nanoparticles are synthesized through chemical methods, having extremely toxic and flammable natures.

Objectives: The aim of the present study was to synthesize silver nanoparticles, through the green method of utilizing Sesamum indicum (S. indicum) extract and to determine the potential antibacterial effects of the product against multi-drug resistant Escherichia coli (E. coli).

Materials and Methods: The formation and characterization of AgNPs (silver nanoparticles) were confirmed by UV-vis spectroscopy, energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD) and transmission electron microscope (TEM). All 30 strains of E. coli were isolated from urine cultures of hospitalized patients (Amir Al-Momenin Hospital, Zabol, South-Eastern Iran) with urinary tract infection, 2011-2012. The minimum inhibitory (MIC) concentrations were investigated by microdilution method.

Results: The results showed that isolated E. coli were resistant to four different antimicrobial agents including ceftazidime (26.6%), cefixime (40%), tetracycline (63.3%) and erythromycin (56.6%). The highest MIC value for produced nano silver in S. indicum seed extract, was 200 ppm, against five isolates of E. coli.

Conclusions: Considering the sufficient antimicrobial activities of nanoparticles tested in this study, they are suggested for enterobacterial infection treatment, especially in hospital environment.

Implication for health policy/practice/research/medical education:

The medicinal plants have recently attracted considerable attention, due to their use for therapeutic purposes, against enteric pathogens.

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